BioAcyl Corp
BioAcyl Corp |
|
 |
 |
 |
|
Ogharandukun, E., Tewolde, W., Damtae, E., & others. (2020). Establishing rules for self-adhesion and aggregation of n-glycan sugars using virus glycan shields. Langmuir : the ACS journal of surfaces and colloids, 36(46), 13769. Added by: Dr. Enrique Feoli (03/07/2021, 14:53) Last edited by: Dr. Enrique Feoli (03/07/2021, 14:54) |
 |
| Resource type: Journal Article DOI: 10.1021/acs.langmuir.0c01953 BibTeX citation key: Ogharandukun2020 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Glycobiology Creators: Damtae, Ogharandukun, others, Tewolde Collection: Langmuir : the ACS journal of surfaces and colloids |
Views: 2/254
|
| Abstract |
|
{The surfaces of cells and pathogens are covered with short polymers of sugars known as glycans. Complex N-glycans have a core of three mannose sugars with distal repeats of N-acetylglucosamine and galactose sugars terminating with sialic acid (SA). Long-range ...}
|
| Notes |
|
The surfaces of cells and pathogens are covered with short polymers of sugars known as glycans. Complex N-glycans have a core of three mannose sugars with distal repeats of N-acetylglucosamine and galactose sugars terminating with sialic acid (SA). Long-range tough and short-range brittle self-adhesions were observed between SA and mannose residues, respectively, in ill-defined artificial monolayers. We investigated if and how these adhesions translate when the residues are presented in N-glycan architecture with SA at the surface and mannose at the core and with other glycan sugars. Two pseudotyped viruses with complex N-glycan shields were brought together in force spectroscopy (FS). At higher ramp rates, slime-like adhesions were observed between the shields, whereas Velcro-like adhesions were observed at lower rates. The higher approach rates compress the virus as a whole, and the self-adhesion between the surface SA is sampled. At the lower ramp rates, however, the complex glycan shield is penetrated and adhesion from the mannose core is accessed. The slime-like and Velcro-like adhesions were lost when SA and mannose were cleaved, respectively. While virus self-adhesion in forced contact was modulated by glycan penetrability, the self-aggregation of the freely diffusing virus was only determined by the surface sugar. Mannose-terminal viruses self-aggregated in solution, and SA-terminal ones required Ca2+ ions to self-aggregate. Viruses with galactose or N-acetylglucosamine surfaces did not self-aggregate, irrespective of whether or not a mannose core was present below the N-acetylglucosamine surface. Well-defined rules appear to govern the self-adhesion and -aggregation of N-glycosylated surfaces, regardless of whether the sugars are presented in an ill-defined monolayer, or N-glycan, or even polymer architecture.
|