BioAcyl Corp
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Jung, S.-H., Lee, W., Park, S.-H., Lee, K.-Y., Choi, Y.-J., & Choi, S., et al. (2020). Diclofenac impairs autophagic flux via oxidative stress and lysosomal dysfunction: Implications for hepatotoxicity. Redox Biology, 37, 101751. Added by: Dr. Enrique Feoli (03/04/2022, 12:54) Last edited by: Dr. Enrique Feoli (03/04/2022, 13:00) |
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| Resource type: Journal Article DOI: 10.1016/j.redox.2020.101751 ID no. (ISBN etc.): 2213-2317 BibTeX citation key: Jung2020 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Chronic NSAIDS Creators: Chang, Choi, Choi, Hong, Jung, Kang, Kim, Lee, Lee, Lee, Park Collection: Redox Biology |
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| Abstract |
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Treatment with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with various side effects, including cardiovascular and hepatic disorders. Studies suggest that mitochondrial damage and oxidative stress are important mediators of toxicity, yet the underlying mechanisms are poorly understood. In this study, we identified that some NSAIDs, including diclofenac, inhibit autophagic flux in hepatocytes. Further detailed studies demonstrated that diclofenac induced a reactive oxygen species (ROS)-dependent increase in lysosomal pH, attenuated cathepsin activity and blocked autophagosome-lysosome fusion. The reactivation of lysosomal function by treatment with clioquinol or transfection with the transcription factor EB restored lysosomal pH and thus autophagic flux. The production of mitochondrial ROS is critical for this process since scavenging ROS reversed lysosomal dysfunction and activated autophagic flux. The compromised lysosomal activity induced by diclofenac also inhibited the fusion with and degradation of mitochondria by mitophagy. Diclofenac-induced cell death and hepatotoxicity were effectively protected by rapamycin. Thus, we demonstrated that diclofenac induces the intracellular ROS production and lysosomal dysfunction that lead to the suppression of autophagy. Impaired autophagy fails to maintain mitochondrial integrity and aggravates the cellular ROS burden, which leads to diclofenac-induced hepatotoxicity.
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| Notes |
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Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli |
